Bull Semen Obtained on Beef Farms by Electroejaculation: Sperm Quality in the First Two Hours of Storing with Different Extenders and Holding Temperatures

  1. Santiago Pernas 1
  2. Aitor Fernández-Novo 1
  3. Clara Barrajon-Masa 2
  4. Patricia Mozas 2
  5. Natividad Pérez-Villalobos 1
  6. Bárbara Martín-Maldonado 1
  7. Agustín Oliet 2
  8. Susana Astiz 3
  9. Sonia S. Pérez-Garnelo 3
  1. 1 Universidad Europea de Madrid
    info

    Universidad Europea de Madrid

    Madrid, España

    ROR https://ror.org/04dp46240

  2. 2 Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario
    info

    Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario

    Madrid, España

  3. 3 Consejo Superior de Investigaciones Científicas
    info

    Consejo Superior de Investigaciones Científicas

    Madrid, España

    ROR https://ror.org/02gfc7t72

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Revista:
Animals

ISSN: 2076-2615

Año de publicación: 2023

Volumen: 13

Número: 9

Páginas: 1561

Tipo: Artículo

DOI: 10.3390/ANI13091561 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Animals

Resumen

Sperm quality decreases over time, so bull semen may need to be preserved after field collection. However, the effect of handling such semen samples from commercial farms and placing them in very short–term storage has not been elucidated. Therefore, ejaculate from 25 bulls from 1 dairy and 14 beef cattle farms were collected under farm conditions and evaluated for semen quality during the first two hours after collection. Two commercial extenders (AndroMed® and BIOXcell®) and two different storage temperatures (5 °C and room temperature) were used to evaluate the influence on semen quality and sperm kinetics in ejaculates grouped into three evaluation times, based on time since collection (Time 1: <75 min, n = 7; Time 2: 75–105 min, n = 11; and Time 3: 105–120 min, n = 7). Classical semen parameters, sperm motion kinetics by CASA and colony-forming units were assessed. The differences between both extenders in curvilinear and straight–line velocities (VCL and VSL) for the different time groups (Time 2 and Time 3) were statistically significant for p < 0.05. AndroMed® showed lower VSL, straightness and linearity in sperm compared to BIOXcell® (p < 0.05). In conclusion, AndroMed® induced more curvilinear movement, while BIOXcell® stimulated straighter motility.

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Información de financiación

Funding: This research received a partial funding of INIA–CSIC (RZP2021-003. Banco de Recursos Zoogenéticos).

Financiadores

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