Co-purification of calcium transport-stimulating and DNA synthesis-stimulating agents with parathormone-like activity isolated from the hypercalcaemic strain of the Walker 256 tumour

  1. F. de Miguel 1
  2. R. García-Cañero 2
  3. P. Esbrit 1
  1. 1 Fundación Jiménez Díaz
    info

    Fundación Jiménez Díaz

    Madrid, España

    ROR https://ror.org/049nvyb15

  2. 2 Hospital Universitario Puerta de Hierro
    info

    Hospital Universitario Puerta de Hierro

    Madrid, España

    ROR https://ror.org/01e57nb43

Revista:
European Journal of Cancer and Clinical Oncology

ISSN: 0277-5379

Año de publicación: 1991

Volumen: 27

Número: 8

Páginas: 1022-1026

Tipo: Artículo

DOI: 10.1016/0277-5379(91)90273-G GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: European Journal of Cancer and Clinical Oncology

Resumen

One of the strains of the Walker 256 carcinosarcoma induces in the rat a humoral hypercalcaemia of malignancy (HHM) syndrome which is similar to that reported in human patients. We have isolated from this tumour a chromatographic fraction which displays an adenylate cyclase stimulating activity in dog kidney cortical membranes, similar to that of a parathormone (PTH) related protein isolated from various HHM related tumours. In addition, this fraction stimulated initial calcium (Ca) uptake in confluent Madin-Darby canine kidney (MDCK) cell monolayers in a dose-dependent manner. Maximal stimulation of Ca uptake was associated with an enhanced Ca efflux from MDCK cells preloaded with the cation, and with an increased DNA synthesis in these cells. These activities might be involved in development of increased tubular calcium reabsorption in Walker 256 tumour-bearing rats.

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