Co-purification of calcium transport-stimulating and DNA synthesis-stimulating agents with parathormone-like activity isolated from the hypercalcaemic strain of the Walker 256 tumour
- F. de Miguel 1
- R. García-Cañero 2
- P. Esbrit 1
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1
Fundación Jiménez Díaz
info
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2
Hospital Universitario Puerta de Hierro
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ISSN: 0277-5379
Ano de publicación: 1991
Volume: 27
Número: 8
Páxinas: 1022-1026
Tipo: Artigo
Outras publicacións en: European Journal of Cancer and Clinical Oncology
Resumo
One of the strains of the Walker 256 carcinosarcoma induces in the rat a humoral hypercalcaemia of malignancy (HHM) syndrome which is similar to that reported in human patients. We have isolated from this tumour a chromatographic fraction which displays an adenylate cyclase stimulating activity in dog kidney cortical membranes, similar to that of a parathormone (PTH) related protein isolated from various HHM related tumours. In addition, this fraction stimulated initial calcium (Ca) uptake in confluent Madin-Darby canine kidney (MDCK) cell monolayers in a dose-dependent manner. Maximal stimulation of Ca uptake was associated with an enhanced Ca efflux from MDCK cells preloaded with the cation, and with an increased DNA synthesis in these cells. These activities might be involved in development of increased tubular calcium reabsorption in Walker 256 tumour-bearing rats.
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