Growth factor ligands of tyrosine kinase receptors activate the Rous sarcoma virus promoter by a Ras- and Raf-dependent mechanism

  1. Cosgaya, Jose Miguel 1
  2. Perez-Juste, German 1
  3. Castillo, Ana Isabel 1
  4. Aranda, Ana 1
  1. 1 Consejo Superior de Investigaciones Científicas
    info

    Consejo Superior de Investigaciones Científicas

    Madrid, España

    ROR https://ror.org/02gfc7t72

Revista:
Gene

ISSN: 0378-1119

Año de publicación: 1997

Volumen: 188

Número: 2

Páginas: 291-293

Tipo: Artículo

DOI: 10.1016/S0378-1119(96)00809-8 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Gene

Resumen

In PC12 cells, NGF and other ligands of tyrosine kinase receptors increased the activity of the Rous sarcoma virus (RSV) promoter. IGF-1 stimulated the RSV promoter in SH-SY5Y neuroblastoma cells. This promoter was also activated by oncogenic Ras and Raf in different cell types, and growth factor induction was inhibited by expression of dominant negative forms of ras and raf, showing that its effect is mediated by a Ras- and Raf-dependent mechanism. Therefore, the RSV promoter should not be used in the determination of transfection efficiency in the studies on regulation of gene expression by ligands of tyrosine kinase receptors, ras and raf.

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Información de financiación

This research was supported by Grants PM92-0002 and PB94-0094 from the DGICYT, by the Comunidad de Madrid and by the Fundación Ramón Areces.

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