Growth factor ligands of tyrosine kinase receptors activate the Rous sarcoma virus promoter by a Ras- and Raf-dependent mechanism
- Cosgaya, Jose Miguel 1
- Perez-Juste, German 1
- Castillo, Ana Isabel 1
- Aranda, Ana 1
-
1
Consejo Superior de Investigaciones Científicas
info
ISSN: 0378-1119
Datum der Publikation: 1997
Ausgabe: 188
Nummer: 2
Seiten: 291-293
Art: Artikel
Andere Publikationen in: Gene
Zusammenfassung
In PC12 cells, NGF and other ligands of tyrosine kinase receptors increased the activity of the Rous sarcoma virus (RSV) promoter. IGF-1 stimulated the RSV promoter in SH-SY5Y neuroblastoma cells. This promoter was also activated by oncogenic Ras and Raf in different cell types, and growth factor induction was inhibited by expression of dominant negative forms of ras and raf, showing that its effect is mediated by a Ras- and Raf-dependent mechanism. Therefore, the RSV promoter should not be used in the determination of transfection efficiency in the studies on regulation of gene expression by ligands of tyrosine kinase receptors, ras and raf.
Informationen zur Finanzierung
This research was supported by Grants PM92-0002 and PB94-0094 from the DGICYT, by the Comunidad de Madrid and by the Fundación Ramón Areces.Geldgeber
- comunidad de madrid Spain
- Fundación Ramón Areces Spain
Bibliographische Referenzen
- Guerrero, I., Pellicer, A. and Burstein, D.E. (1988) Dissociation of c-Fos from ODC expression in a PC12 subline stably transfected with an inducible N-ras oncogene. Biochem. Biophys. Res. Commun. 150, 1585–1592.
- Lang, A., Fincham, V.J. and Wyke, J.A. (1993) Factors influencing physiological variations in the activity of the Rous sarcoma virus long terminal repeat. Virology 196, 564–575.
- Saatcioglu, F., Deng, T. and Karin, M. (1993) A novel cis element mediating ligand-independent activation by c-ErbA: implications for hormonal regulation. Cell 75, 1095–1105.
- Schlessinger, J. (1993) How receptor tyrosine kinases activate Ras. Trends Biochem. Sci. 18, 273–275.
- Szeberényi, J., Cai, H. and Cooper, G.M. (1990) Effect of a dominant inhibitory Ha-ras mutation on neuronal differentiation of PC12 cells. Mol. Cell. Biol. 10, 5324–5332.