Involvement of chromatin and histone deacetylation in SV40T antigen transcription regulation

  1. Ester Valls 1
  2. Noemí Blanco-García 1
  3. Naiara Aquizu 1
  4. David Piedra 2
  5. Conchi Estarás 1
  6. Xavier de la Cruz 2
  7. María Ángeles Martínez Balbás 1
  1. 1 Instituto de Biología Molecular de Barcelona
    info

    Instituto de Biología Molecular de Barcelona

    Barcelona, España

    ROR https://ror.org/05t8khn72

  2. 2 IRB Barcelona - Institute for Research in Biomedicine
    info

    IRB Barcelona - Institute for Research in Biomedicine

    Barcelona, España

    ROR https://ror.org/01z1gye03

Revista:
Nucleic Acids Research

Año de publicación: 2007

Volumen: 6

Páginas: 1958-1968

Tipo: Artículo

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Resumen

Simian Virus 40 (SV40) large T antigen (T Ag) is amultifunctional viral oncoprotein that regulatesviral and cellular transcriptional activity. However,the mechanisms by which such regulation occursremain unclear. Here we show that T antigenrepresses CBP-mediated transcriptional activity.This repression is concomitant with histone H3deacetylation and is TSA sensitive. Moreover,our results demonstrate that T antigen interactswith HDAC1 in vitro in an Rb-independent manner.In addition, the overexpression of HDAC1 cooperates with T antigen to antagonize CBP transactivation function and correlates with chromatindeacetylation of the TK promoter. Finally, decreasing HDAC1 levels with small interfering RNA (siRNA)partially abolishes T antigen-induced repression.These findings highlight the importance of thehistone acetylation/deacetylation balance in thecellular transformation mediated by oncoviralproteins.

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