Regulation of Androgen and Vitamin D Receptors by 1,25-Dihydroxyvitamin D3 in Human Prostate Epithelial and Stromal Cells

  1. LEMAN, EDDY S. 1
  2. Miguel Pedrero, Fernando de 1
  3. GAO, ALLEN C. 1
  4. GETZENBERG, ROBERT H. 1
  1. 1 University of Pittsburgh
    info

    University of Pittsburgh

    Pittsburgh, Estados Unidos

    ROR https://ror.org/01an3r305

Revista:
Journal of Urology

ISSN: 0022-5347,  1527-3792

Año de publicación: 2003

Volumen: 170

Número: 1

Páginas: 235-240

Tipo: Artículo

DOI: 10.1097/01.JU.0000060117.61770.35 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Journal of Urology

Resumen

Purpose:The mechanisms of the interaction between 1,25-dihydroxyvitamin D3 (1,25 D) and androgens, and their respective receptors in their action on the prostate are not completely understood. We examined the interplay of 1,25 D and androgens on the epithelial and stromal cells of the prostate.Materials and Methods:The human neonatal prostatic epithelial cell line 267B-1 (BRFF, Inc., Ijamsville, Maryland) and primary cultures of human prostate stromal cells were treated with medium containing 5 or 10 μM 1,25 D or ethanol (control) in the presence or absence of 10 nM dihydrotestosterone (DHT) (Sigma Chemical Co., St. Louis, Missouri). Protein levels of androgen receptor (AR) and vitamin D receptor (VDR) were determined by immunoblot analysis of whole cell extracts. Electrophoresis mobility shift assays were used to determine AR and VDR DNA binding activities.Results:The VDR protein level of 267B-1 cells was increased in the presence of 1,25 D (with the maximum effects seen at 24 hours) regardless of the presence or absence of DHT. In addition, exogenous DHT increased the AR and VDR DNA binding activities of 267B-1 and stromal cells in the presence of 1,25 D.Conclusions:ARs in the normal prostate are regulated by androgens, whereas VDRs in the normal prostate can be regulated by 1,25 D as well as by other androgens such as testosterone. This finding further supports the concept that 1,25 D as a steroid hormone, in addition to other androgens such as DHT, may have a role in the growth and differentiation of normal prostate.

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