Isolation, Survival, Proliferation, and Differentiation of Human Neural Stem Cells

  1. Navarro, Beatriz
  2. Villa, Ana
  3. Liste, Isabel
  4. Bueno, Carlos
  5. Martínez-Serrano, Alberto
Libro:
Neural Stem Cells

ISBN: 140207588X

Año de publicación: 2003

Páginas: 271-298

Tipo: Capítulo de Libro

DOI: 10.1007/0-306-48356-4_9 GOOGLE SCHOLAR lock_openAcceso abierto editor

Objetivos de desarrollo sostenible

Resumen

Neural stem cells are essential cellular elements for nervous system generation and maintenance (Anderson, 2001; Temple, 2001). During the last decade, an impressive amount of information has been generated regarding thebasic in vitro and in vivo biology of neural stem cells (NSCs). A quick search inpublic databases on terms like survival, proliferation, and differentiation of NSCsimmediately retrieves thousands of research articles and reviews published inthe last two years on these topics. Although the majority of this research, particularly that one dealing with genetic analyses and behavior of NSCs in situations of neurodegeneration in vivo, has been obviously conducted in rodents, agrowing knowledge about human NSCs (hNSCs) biology is also rapidly becoming available. The main objective of this chapter will be to summarize recent advances in our understanding of the biology of hNSCs, particularly inthose aspects related to translation of basic research to potential therapeuticapplications. We, in advance would like to apologize to many colleagues whosework can not be summarized here due to space constraints. Whenever needed,and due to the absence of information in the human setting in many respects,research in rodent systems will also be summarized. This chapter will be organized in a few sections aimed to cover isolation procedures of hNSCs, culturingor proliferation methods, and properties of the different cellular systems, factors influencing survival and differentiation (two aspects intimately linked),and finish with an account of recent transplantation experiments illustrating thesurvival, migration and differentiation capabilities of hNSCs when grafted intothe fetal, neonatal and adult rodent and primate brain

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