Sequence of the Streptococcus pneumoniae bacteriophage HB-3 amidase reveals high homology with the major host autolysin

  1. Romero, A 1
  2. Lopez, R 1
  3. Garcia, P 1
  1. 1 Centro de Investigaciones Biológicas
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    Centro de Investigaciones Biológicas

    Madrid, España

    ROR https://ror.org/04advdf21

Revista:
Journal of Bacteriology

ISSN: 0021-9193,  1098-5530

Año de publicación: 1990

Volumen: 172

Número: 9

Páginas: 5064-5070

Tipo: Artículo

DOI: 10.1128/JB.172.9.5064-5070.1990 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Journal of Bacteriology

Resumen

We have sequenced a DNA fragment containing the pneumococcal bacteriophage HB-3 hbl gene, which codes for the phage lytic amidase. A remarkable nucleotide similarity (87.1%) between the lytA gene, coding for the pneumococcal amidase, the major autolysin of Streptococcus pneumoniae, and the hbl gene was found. This similarity completely disappeared outside the open reading frames coding for both amidases. The hbl gene transformed amidase-deficient strains of S. pneumoniae to the wild-type phenotype, and Southern blotting experiments provided evidence for recombination between donor and recipient genes. A comprehensive evaluation of these and previous results on the peptidoglycan hydrolases of S. pneumoniae and its bacteriophages suggested that recombination mechanisms participate in the evolution of the genes coding for these enzymes.

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Información de financiación

This work was supported by grant PB87-0214 from Dirección General de Investigación Científica y Técnica. A.R. was the recipient of a fellowship from Plan de Formación del Personal Investigador.

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