The insertion site of the temperate phage HB-746 is located near the phage remnant in the pneumococcal host chromosome

  1. Romero, A 1
  2. Lopez, R 1
  3. Garcia, P 1
  1. 1 Centro de Investigaciones Biológicas
    info

    Centro de Investigaciones Biológicas

    Madrid, España

    ROR https://ror.org/04advdf21

Revista:
Journal of Virology

ISSN: 0022-538X,  1098-5514

Año de publicación: 1992

Volumen: 66

Número: 5

Páginas: 2860-2864

Tipo: Artículo

DOI: 10.1128/JVI.66.5.2860-2864.1992 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Journal of Virology

Resumen

Combined Southern blot hybridization analyses of DNA digests of several clinical strains of Streptococcus pneumoniae have revealed the presence of a gene (hblR), or part of it, similar to the hbl7 gene coding for the cell wall lytic enzyme of the temperate HB-746 phage. The results confirmed that the genome of HB-746, which contains protein covalently linked to the 5' ends of its DNA, becomes integrated into the host strain 8R1 and showed that both the host and phage attachment sites, attB and attP, lie downstream of the 3' end of the structural region of the hblR and hbl7 genes, respectively. The data reported also highlight some evolutionary relationships between phage and bacteria.

€ Ver financiación

Información de financiación

This work was supported by a grant from Dirección General de Investigación Científica y Técnica (PB90-0069). A.R. was the recipient of a fellowship from Plan de Formación del Personal Investigador

Financiadores

Referencias bibliográficas

  • 1.Anilionis,A.,andM.Riley.1980.Conservationandvariationofnucleotidesequenceswithinrelatedbacterialgenomes:Esche-richiacolistrains.J.Bacteriol.143:355-365.
  • 2.Bernheimer,H.P.1979.Lysogenicpneumococciandtheirbacteriophages.J.Bacteriol.138:618-624.
  • 3.Campbell,A.1988.Phageevolutionandspeciation,p.1-14.InR.Calendar(ed.),Thebacteriophages,vol.1.PlenumPublish-ingCorp.,NewYork.
  • 4.Craig,N.L.1988.Themechanismofconservativesite-specificrecombination.Annu.Rev.Genet.22:77-105.
  • 5.Dowson,C.G.,A.Hutchinson,J.A.Brannigan,R.C.George,D.Hansman,J.Lifiares,A.Tomasz,J.Maynard-Smith,andB.G.Spratt.1989.Horizontaltransferofpenicillin-bindingproteingenesinpenicillin-resistantclinicalisolatesofStrep-tococcuspneumoniae.Proc.Natl.Acad.Sci.USA86:8842-8846.
  • 6.Garcia,P.,J.L.Garcia,E.Garcia,andR.L6pez.1986.Nucleotidesequenceandexpressionofthepneumococcalau-tolysingenefromitsownpromoterinEscherichiacoli.Gene43:265-272.
  • 7.Garcia,P.,J.L.Garcia,E.Garcia,J.M.Sanchez-Puelles,andR.L6pez.1990.ModularorganizationofthelyticenzymesofStreptococcuspneumoniaeanditsbacteriophages.Gene86:81-88.
  • 8.Gasc,A.M.,L.Kauc,P.Barraille,M.Sicard,andS.Goodgal.1991.Genelocalization,size,andphysicalmapofthechromo-someofStreptococcuspneumoniae.J.Bacteriol.173:7361-7367
  • 9.Inciarte,M.R.,J.M.Lazaro,M.Salas,andE.Vifiuela.1976.Physicalmapofbacteriophage<b29DNA.Virology74:1314-1323.
  • 10.Laible,G.,B.G.Spratt,andR.Hakenbeck.1991.InterspeciesrecombinationaleventsduringtheevolutionofalteredPBP2xgenesinpenicillin-resistantclinicalisolatesofStreptococcuspneumoniae.Mol.Microbiol.5:1993-2002.
  • 11.Lee,C.Y.,andJ.J.Iandolo.1986.IntegrationofstaphylococcalphageL54aoccursbysite-specificrecombination:structuralanalysisoftheattachmentsites.Proc.Natl.Acad.Sci.USA83:5474-5478.
  • 12.Lee,C.Y.,andJ.J.Iandolo.1988.Structuralanalysisofstaphylococcalbacteriophage411attachmentsites.J.Bacte-riol.170:2409-2411.
  • 13.Moynet,D.J.,andG.J.Tiraby.1980.Inhibitionoftransforma-tioninStreptococcuspneumoniaebylysogeny.J.Bacteriol.141:1298-1304.
  • 14.Muckerman,C.C.,S.S.Springhorn,B.Greenberg,andS.A.Lacks.1982.Transformationofrestrictionendonucleasepheno-typeinStreptococcuspneumoniae.J.Bacteriol.152:183-190.
  • 15.Pierson,L.S.,III,andM.L.Kahn.1987.IntegrationofsatellitebacteriophageP4inEscherichiacoli.DNAsequencesofthephageandhostregionsinvolvedinsite-specificrecombination.VOL.66,1992 J.Mol.Biol.196:487-496.
  • 16.Reanney,D.C.,andH.W.Ackermann.1982.Comparativebiologyandevolutionofbacteriophages.Adv.VirusRes.27:205-280.
  • 17.Romero,A.,R.L6pez,andP.Garcia.1990.CharacterizationofthepneumococcalbacteriophageHB-3amidase:cloningandexpressioninEscherichiacoli.J.Virol.64:137-142.
  • 18.Romero,A.,R.Lopez,andP.Garcia.1990.SequenceoftheStreptococcuspneumoniaebacteriophageHB-3amidasere-vealshighhomologywiththemajorhostautolysin.J.Bacteriol.172:5064-5070.
  • 19.Romero,A.,R.L6pez,R.Lurz,andP.Garcia.1990.TemperatebacteriophagesofStreptococcuspneumoniaethatcontainpro-teincovalentlylinkedtothe5'endsoftheirDNA.J.Virol.64:5149-5155.
  • 20.Sambrook,J.,E.F.Fritsch,andT.Maniatis.1989.Molecularcloning:alaboratorymanual.ColdSpringHarborLaboratory,ColdSpringHarbor,N.Y.
  • 21.Southern,E.M.1975.DetectionofspecificsequencesamongDNAfragmentsseparatedbygelelectrophoresis.J.Mol.Biol.98:503-517.
  • 22.Stroynowski,I.T.1981.Distributionofbacteriophage+3ThomologousdeoxyribonucleicacidsequencesinBacillussub-tilis168,relatedbacteriophages,andotherBacillusspecies.J.Bacteriol.148:91-100.
  • 23.Waldman,A.S.,S.D.Goodman,andJ.J.Scocca.1987.NucleotidesequencesandpropertiesofthesitesinvolvedinlysogenicinsertionofthebacteriophageHP1c1genomeintotheHaemophilusinfluenzaechromosome.J.Bacteriol.169:238-246.
  • 24.Weisberg,R.A.,andA.Landy.1983.Site-specificrecombina-tioninphagelambda,p.211-250.InR.W.Hendrix,J.W.Roberts,F.W.Stahl,andR.A.Weisberg(ed.),Lambda1I.ColdSpringHarborLaboratory,ColdSpringHarbor,N.Y.J.VIROL.